Production of dumbbell probe through hairpin cleavage-ligation and increasing RCA sensitivity and specificity by circle to circle amplification
نویسندگان
چکیده
منابع مشابه
Production of dumbbell probe through hairpin cleavage-ligation and increasing RCA sensitivity and specificity by circle to circle amplification
Dumbbell probe (DP) attracts increasing interests in rolling circle amplification (RCA). A universal DP production method through cleavage-ligation of hairpin was proposed and optimized. The production is characterized by restriction endonuclease (RE)-induced cleavage ends ligation. It has the advantage of phosphorylation-free, splint-free and purification-free. To optimize designing, we found ...
متن کاملRolling Circle Amplification (RCA): an approach for quick detection and identification of fungal species
Conventional methods for fungal identification in the clinical laboratory rely on morphological andphysiological tests. These tests often need several days or weeks to complete and are frequentlyunspecific. Molecular identification mostly implies sequencing, which is relatively expensive andtime-consuming, as well as impractical for large numbers of isolates. The Rolling CircleAmplification app...
متن کاملrolling circle amplification (rca): an approach for quick detection and identification of fungal species
conventional methods for fungal identification in the clinical laboratory rely on morphological andphysiological tests. these tests often need several days or weeks to complete and are frequentlyunspecific. molecular identification mostly implies sequencing, which is relatively expensive andtime-consuming, as well as impractical for large numbers of isolates. the rolling circleamplification app...
متن کاملA dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection
We herein report the design of a dumbbell-shaped DNA probe that integrates target-binding, amplification and signaling within one multifunctional design. The dumbbell probe can initiate rolling circle amplification (D-RCA) in the presence of specific microRNA (miRNA) targets. This D-RCA-based miRNA strategy allows quantification of miRNA with very low quantity of RNA samples. The femtomolar sen...
متن کاملCircle-to-circle amplification for precise and sensitive DNA analysis.
We present a tightly controlled process for strand-specific amplification of circularized DNA molecules. Tandem repeated complements of DNA circles are generated by rolling-circle replication, and converted to monomer circles of opposite polarity to that of the starting material. These circles are then subjected to one more round of rolling-circle replication and circularization, and the proces...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Scientific Reports
سال: 2016
ISSN: 2045-2322
DOI: 10.1038/srep29229